DNA fingerprinting discord

A DNA Fingerprinting Exercise for

"Hands on" laboratory exercises are excellent teaching tools because they have the potential to reach students with all types of learning styles. Unfortunately the large monetary investment required to obtain supplies and equipment and train instructors prevents many schools from providing hands-on learning experiences with DNA technology. Students in non-laboratory courses are even less likely...

DNA Fingerprinting and RFLP Analysis

Automated Image Analysis for Dna Fingerprinting

Methods for automated detection of DNA restriction fragments resolved on agarose fingerprinting gels are described. We present a mathematical model for the location and shape of the restriction fragments as a function of fragment size, with model parameters determined empirically from marker lanes containing molecular size standards. Automated identification of restriction fragments involves se...

The similarity index and DNA fingerprinting.

DNA-fingerprint similarity is being used increasingly to make inferences about levels of genetic variation within and between natural populations. It is shown that the similarity index--the average fraction of shared restriction fragments--provides upwardly biased estimates of population homozygosity but nearly unbiased estimates of the average identity-in-state for random pairs of individuals....

Multilocus Dna Fingerprinting, Based on A

single probe, have been shown to reflect genetic relatedness accurately in the redwinged blackbird (Agelaius phoeniceus). We maintain that our application of this method in testing for differences in the average level of relatedness between groups is justified. We agree with Griffith and Montgomerie that alternative explanations may need to be considered before concluding that mate choice is ba...